Given below are the different steps in recombinant DNA technology. Arrange them according to the sequence of occurrence .
a. Transferring the recombinant DNA into the host
b. Extraction of the product
c. Fragmentation of DNA by restriction endonucleases
d. Ligation of DNA fragment into a vector
e. Isolation of DNA
f. Culturing the host cells in a medium at large scale .
g. Isolation of a desired DNA fragment .

Explanation

a. Isolation of DNA
b. Fragmentation of DNA by restriction endonucleases
c. Isolation of a desired DNA fragment.
d. Ligation of DNA fragment into a vector
e. Transferring the recombinant DNA into the host .
f. Culturing the host cells in a medium at large scale .
g. Extraction of the product .