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(a) A recombinant vector with a gene of ...

(a) A recombinant vector with a gene of interest inserted within the gene of beta-galactosidase enzyme, is introduced into a bacterium. Explain the method that would help in selection of recombinant colonies from non-recombinant ones.
(b) Why is this method of selection referred to as ''insertional inactivation''?

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### Step-by-Step Solution: **(a)** To select recombinant colonies from non-recombinant ones using a recombinant vector that contains a gene of interest inserted within the gene of beta-galactosidase, we can utilize a method based on the activity of the beta-galactosidase enzyme. Here’s how the process works: 1. **Introduction of the Vector**: A recombinant vector containing the gene of interest is introduced into a bacterial host (commonly E. coli). This vector also contains the gene for the beta-galactosidase enzyme. 2. **Transformation**: The bacteria take up the recombinant vector through a process called transformation. However, not all bacteria will successfully incorporate the vector; some will remain untransformed or take up non-recombinant vectors. ...
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