List the two methodologies which were involved in human genome project . Mention how they were used.
Explain YAC and mention what was it used for.

Explanation

### Step-by-Step Solution:

1. **Identify the Two Methodologies Used in the Human Genome Project (HGP)**:
- The two primary methodologies involved in the Human Genome Project are:
- **Expressed Sequence Tags (EST)**
- **Sequence Annotation**

2. **Explain How Each Methodology Was Used**:
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