During in vitro synthesis of DNA, a researcher used 2. 3' dideoxy cytidine triphosphate as raw nucleotide in place of 2 deoxy cytidine triphosphate, other conditions remaining as standard. will further polymerisation of DNA continue upto the end or not? Explain.

Explanation

To determine whether further polymerization of DNA will continue when using 2,3-dideoxycytidine triphosphate (ddCTP) instead of 2-deoxycytidine triphosphate (dCTP), we need to understand the role of these nucleotides in DNA synthesis.

### Step-by-Step Solution:

1. **Understanding Nucleotide Structure**:
- Nucleotides are the building blocks of DNA, consisting of a sugar, a phosphate group, and a nitrogenous base.
- 2-deoxycytidine triphosphate (dCTP) has a hydroxyl (-OH) group at the 3' position of the sugar, which is essential for forming phosphodiester bonds during DNA polymerization.

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