Plant part used for tissue culture is sterilized by

Options

A. Chemicals
B. Heat
C. UV-rays
D. Nutrient media

Answer

### Step-by-Step Solution:

1. **Understanding Tissue Culture**:
- Tissue culture refers to the growth of plant tissues or cells in an artificial medium. This method is widely used in plant propagation and genetic engineering.

2. **Importance of Sterilization**:
- Before introducing plant parts into the tissue culture medium, it is essential to sterilize them to eliminate any potential contaminants such as bacteria and fungi.

3. **Methods of Sterilization**:
- There are various methods to sterilize plant parts, including the use of chemicals, heat, UV rays, and nutrient media.

4. **Identifying the Correct Method**:
- Among the options provided:
- **Chemicals**: Commonly used chemicals for sterilization include ethyl alcohol and sodium hypochlorite.
- **Heat**: While heat can be used, it is not the primary method for sterilizing plant tissues in tissue culture.
- **UV Rays**: UV rays can be used for sterilization but are not the most common method for plant tissues.
- **Nutrient Media**: Nutrient media is used for growing the tissues but is not a sterilization method.

5. **Conclusion**:
- The correct answer is that the plant part used for tissue culture is sterilized by **chemicals**.

### Final Answer:
The plant part used for tissue culture is sterilized by **chemicals**.

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