Denaturation of protein can be brought about by

Options

A. Changing concentration
B. Changing pH
C. Changing Temperature
D. Changing amino acid sequence

Answer

**Step-by-Step Solution:**

1. **Understanding Denaturation**: Denaturation of proteins refers to the process where the protein loses its native structure without breaking its primary structure (the sequence of amino acids). This loss of structure leads to a loss of biological activity.

2. **Identifying Factors Causing Denaturation**: The question provides four options: changing concentration, changing pH, changing temperature, and changing amino acid sequence. We need to determine which of these can cause denaturation.

3. **Analyzing Each Option**:
- **Option A: Changing Concentration**: Changing the concentration of the protein does not typically lead to denaturation. Therefore, this option is incorrect.
- **Option B: Changing pH**: Altering the pH can disrupt the ionic and hydrogen bonds that maintain the protein's structure, leading to unfolding of the protein. This is a correct option.
- **Option C: Changing Temperature**: Increasing temperature raises the kinetic energy of molecules, which can disrupt weak interactions (like hydrogen bonds and van der Waals forces) that stabilize the protein structure. This is also a correct option.
- **Option D: Changing Amino Acid Sequence**: Changing the amino acid sequence alters the primary structure of the protein, which is not considered denaturation. Thus, this option is incorrect.

4. **Conclusion**: The correct answers are options B (changing pH) and C (changing temperature) as both can lead to the denaturation of proteins.

**Final Answer**: Denaturation of protein can be brought about by changing pH (Option B) and changing temperature (Option C).

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