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Assertion : Restriction enzymes cut the ...

Assertion : Restriction enzymes cut the strand of DNA to produce sticky ends.
Reason : Stickness of the ends facilitates the action of the enzyme DNA polymerase

A

if both are true with reason being correct explanation

B

both true but reason is not correct explanation

C

assertion true but reason is wrong

D

both are wrong

Text Solution

AI Generated Solution

The correct Answer is:
**Step-by-Step Solution:** 1. **Understanding the Assertion:** - The assertion states that restriction enzymes cut the DNA strand to produce sticky ends. - Restriction enzymes are proteins that recognize specific sequences in DNA and cut the DNA at those sites. - When they cut, they can leave overhanging ends, known as sticky ends, which are crucial for DNA recombination. 2. **Understanding the Reason:** - The reason provided states that the stickiness of the ends facilitates the action of the enzyme DNA polymerase. - DNA polymerase is an enzyme that synthesizes new DNA strands by adding nucleotides to a growing chain, but it does not utilize sticky ends for its action. 3. **Analyzing the Correctness of the Reason:** - The assertion is true; restriction enzymes do produce sticky ends. - However, the reason is incorrect because it incorrectly attributes the function of sticky ends to DNA polymerase instead of DNA ligase. - DNA ligase is the enzyme that actually joins DNA fragments with sticky ends. 4. **Conclusion:** - Therefore, the assertion is true, but the reason is false. The correct relationship is that sticky ends facilitate the action of DNA ligase, not DNA polymerase. 5. **Final Answer:** - The answer is that the assertion is true, but the reason is false. This corresponds to option 3. ---
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