You are provided with the mixture of transformed bacteria having recom-binant plasmids in which a foreign DNA is linked at the sits of tetracy- cline resistance and non transformed bacteria . How will you select the recombinant plasmids from the mixture ?

Explanation

The recombinant plasmids will lose tetracycline resistance due to the insertion of foreign DNA . Normal plasmids will grow both in tetracycline containing medium and ampicillin medium. The bacterial cells are plated first on ampicillin containing medium and then on tetracycline contain-ing medium. The transformants don not grow on tetracyline containing medium. But the nontransformants will grow on both medium .